BB10186: Proteins: purification and characterisation
[Page last updated: 02 August 2022]
Academic Year: | 2022/23 |
Owning Department/School: | Department of Biology & Biochemistry |
Credits: | 6 [equivalent to 12 CATS credits] |
Notional Study Hours: | 120 |
Level: | Certificate (FHEQ level 4) |
Period: |
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Assessment Summary: | EX 75%, MC 25% |
Assessment Detail: |
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Supplementary Assessment: |
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Requisites: |
Before taking this module you must take BB10003 AND take CH10088
While taking this module you must take BB10121 AND take CH10087 |
Learning Outcomes: | After taking this unit the student should be able to:
* Describe common methods for purifying proteins and be able to design a simple protein purification scheme; * Describe the use of different spectroscopic techniques to probe structural features of proteins; * Describe physical techniques used to elucidate the function and oligomeric state of proteins. |
Aims: | To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins. |
Skills: | Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F. |
Content: | Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins. |
Programme availability: |
BB10186 is Compulsory on the following programmes:Department of Biology & Biochemistry
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Notes:
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